Assessing Genome Accessibility and the Impact on Microbial Factory Performance Using CRISPR-based Genome Editing Tool

CATEGORIES: Uncategorized

Course: ChE 490/ Micro 490/Biol 499
Credits: 1-2
Instructor: Dr. Zengyi Shao

Genomes serve as scaffolds for transmitting information through both genetic and epigenetic means. Eukaryotes face an information packaging challenge because DNA molecules of each chromosome need to be folded within a tiny space in the nuclei. Accumulating evidence demonstrates that the spatial arrangement of the genomes in eukaryotes is far from random. We are interested in studying the influence offered by different genome contexts on heterologous pathway performance, which is directly correlated to the productivity when the host is engineered as a microbial factory to produce high-value chemicals (e.g., biofuels, biopolymer precursors and other compounds used as nutraceuticals and pharmaceuticals). Through this two-semester vertically integrated program (VIP), students will be exposed to frontier research topics such as high throughput DNA assembly and CRISPR-based genome editing technology, and also learn the techniques to generate recombinant DNAs, perform flow cytometry, and gene knock-in/knock-out.

To participate in this project, contact Dr. Zengyi Shao

Topics: Synthetic biology for students in Chemical Engineering, Microbiology, Biochemistry, Biology, or Genetics